1. Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China;
2. Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, China;
3. Institute of Biology, Westlake Institute for Advanced Study, Hangzhou 310024, China;
Shi’s group has been focusing on the structural and mechanistic elucidation of pre-mRNA splicing in the past decades. After the success of crystal structural elucidation of subcomplexes within spliceosome
which conceptionally advance our understanding to the molecular mechanism of pre-mRNA splicing and provide unprecedented insights into the research on pathogenesis of related diseases and drug discovery.
they took a direct assault on the intact spliceosome and made the breakthrough in the summer of 2015. They determined the structure of intron lariat spliceosome ILS complex from S. pombe at an average resolution of 3.6 Å