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SCIENCE CHINA Life Sciences, Volume 63 , Issue 3 : 450-452(2020) https://doi.org/10.1007/s11427-019-1630-2

ScCas9 recognizes NNG protospacer adjacent motif in genome editing of rice

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  • ReceivedDec 23, 2019
  • AcceptedJan 14, 2020
  • PublishedJan 17, 2020

Abstract

There is no abstract available for this article.


Funded by

the National Transgenic Science and Technology Program(2019ZX08010-001,2019ZX08010-003,2016ZX08001004-002)

the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.


Acknowledgment

This work was supported by the National Transgenic Science and Technology Program (2019ZX08010-001, 2019ZX08010-003 and 2016ZX08001004-002) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.


Interest statement

The author(s) declare that they have no conflict of interest.


Supplement

SUPPORTING INFORMATION

Figure S1 Complete DNA sequences of the rice codon-optimized ScCas9.

The supporting information is available online at http://life.scichina.com and https://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.


References

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  • Figure 1

    Genome editing in rice using SpCas9, ScCas9 and SpCas9-LK variants. A, Schematic illustration of ScCas9 and SpCas9-LK variants. U3, U3 promoter; NLS, nuclear localization signal; CaMV T, cauliflower mosaic virus 35S terminator; OsActin P, OsActin promoter. Loop (367–376, IKHRKRTTKL) and KQ (1337–1338) are two important insertions in ScCas9. B, Mutation frequency of SpCas9, ScCas9 and SpCas9-LK at NNG PAM target sites in transgenic calli and mutation frequency of ScCas9 in seedlings. The PAM motif is in red and underlined, and the flanking sequence around the PAM motif is in blue. Bi, biallele; He, heterozygote; Chi, Chimera.

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