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SCIENCE CHINA Life Sciences, Volume 63 , Issue 8 : 1159-1167(2020) https://doi.org/10.1007/s11427-019-1571-8

FET-based nanobiosensors for the detection of smell and taste

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  • ReceivedSep 9, 2019
  • AcceptedOct 26, 2019
  • PublishedJan 21, 2020

Abstract


Interest statement

The author(s) declare that they have no conflict of interest.


References

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  • Figure 1

    Scheme of nanobiosensors composed of olfactory/taste receptors and nanomaterials.

  • Figure 2

    Schematics of nanobiosensors showing olfactory receptor based biomaterials that are immobilized on nanomaterials. OR, olfactory receptor.

  • Figure 3

    Strategies to utilize human taste receptors as biomaterials for developing nanobiosensors. A, Schematic representation of the construction of nanovesicles including hTAS1R1/hTAS1R3 or hTAS2R1/hTAS1R3 and the integration of nanovesicles with nanomaterials for detecting umami or sweet tastes. B, Schematic diagram of an alternative approach to utilize VFTs of hTAS1R1 possibly produced in bacterial expression system, and integration of VFTs with nanomaterials for detecting umami tastes. VFT, venus flytrap; CRD, cysteine rich domain; TMD, transmembrane domain.

  • Table 1   Table 1 Natural biomaterial-based nanobiosensors that detect various odorsa)

    Receptors

    Types

    Transducers

    Targets (phase)

    Odors

    LOD

    (limit of detection)

    Refs

    TAAR13c

    Nanodisc

    CNT-FET*

    Cadaverine

    (liquid)

    Foul

    10 pmol L–1

    (Yang et al., 2017)

    hOR1A2

    Nanodisc

    CNT-FET

    Geraniol, citronellol (liquid)

    Floral

    1 fmol L–1,10 fmol L–1

    (Lee et al., 2018)

    olfactory receptor-derived peptide

    Peptide

    CNT-FET

    Trimethylamine (liquid, gas)

    Fishy

    10 fmol L–1,100 ppt

    (Lim et al., 2013), (Lee et al., 2015)

    odorant binding protein-derived peptide

    Peptide

    CNT-FET

    3-methyl-1-butanol(liquid)

    Whiskey

    1 fmol L–1

    (Son et al., 2016)

    hOR1J2

    Nanovesicle

    CNT-FET

    Heptanal

    (liquid)

    Fatty

    10 fmol L–1

    (Lim et al., 2014)

    hOR3A4, hOR51S1

    Nanovesicle

    CNT-FET

    Geosmin,

    2-methylisoborneol (liquid)

    Earthy, musty

    10 ng L–1

    (Son et al., 2015)

    hOR3A1

    Recombinant protein

    CVD graphene*

    Helional

    (liquid, gas)

    Floral

    0.1 fmol L–1,0.02 ppt

    (Kwon et al., 2015), (Lee et al., 2012b)

    CPNT-FET*

    hOR2AG1

    Recombinant protein

    CPNT-FET

    Amyl butyrate (liquid)

    Fruity

    400 fmol L–1

    (Yoon et al., 2009)

    CVD graphene

    0.04 fmol L–1,0.01 fmol L–1

    (Park et al., 2012b), (Kwon et al., 2015)

    CNT-FET

    100 fmol L–1,1 fmol L–1

    (Kim et al., 2009),

    (Lee et al., 2012a)

    Nanovesicle

    CNT-FET

    1 fmol L–1

    (Jin et al., 2012), (Lim et al., 2015)

    hOR2J2

    Detergent micelle

    CNT-FET

    Octanol

    (liquid)

    Soap

    1 pmol L–1

    (Son et al., 2017a)

    hOR2W1

    Detergent micelle

    CNT-FET

    Hexanal

    (liquid)

    Fruity

    1 pmol L–1

    cfOR5269

    Nanovesicle

    1 fmol L–1

    (Park et al., 2012a)

    *, CNT-FET, carbon nanotube-field effect transistor; CPNT, conducting polymer nanotube; CVD, chemical vapor deposition.

  • Table 2   Table 2 Human taste receptor-based nanobioelectronic tongue detecting bitter, umami, and sweet tastea)

    Taste

    Receptors

    Types

    Transducers

    Targets

    LOD

    (limit of detection)

    Refs

    Bitter

    hTAS2R38

    Recombinant protein

    CPNT-FET*

    PTC*, PROP*

    1 fmol L–1,10 fmol L–1

    (Song et al., 2013)

    Recombinant protein

    CNT-FET*

    PTC, PROP

    100 fmol L–1

    (Kim et al., 2011)

    Recombinant protein (detergent micelle)

    CNT-FET

    Goitrin

    1 pmol L–1

    (Son et al., 2017a)

    Umami

    &

    sweet

    hTAS1R2/hTAS1R3

    Nanovesicle

    CNT-FET

    Sucrose

    500 µmol L–1

    (Song et al., 2014)

    hTAS1R1/hTAS1R3, hTAS1R2/hTAS1R3

    Nanovesicle

    CVD graphene-FET*

    MSG*, sucrose

    100 nmol L–1,1 μmol L–1

    (Ahn et al., 2016)

    Umami

    VFT* domain of T1R1

    Recombinant protein (detergent micelle)

    CVD graphene-FET

    MSG

    1 nmol L–1

    (Ahn et al., 2018)

    *, CNT-FET, carbon nanotube-field effect transistor; CPNT, conducting polymer nanotube; CVD, chemical vapor deposition; MSG, monosodium glutamate; PROP, propylthiouracil; PTC, phenylthiocarbamide; VFT, venus flytrap.

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