Chinese Science Bulletin, Volume 62 , Issue 33 : 3851-3860(2017) https://doi.org/10.1360/N972017-00419

In vitro synthetic biology: Cell-free protein synthesis

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  • ReceivedApr 18, 2017
  • AcceptedAug 31, 2017
  • PublishedSep 27, 2017


Contributions statement


Equally contributed to this work


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  • Figure 1

    (Color online) Evolution of the protein synthesis from cell factory system to CFPS

  • Figure 2

    (Color online) The development history of CFPS

  • Figure 3

    (Color online) Overview of CFPS system: production formats, category of cell extracts, category of proteins produced and application fields

  • Table 1   Pros and cons of four types of cell extracts





    蛋白产量高、提取物制备简便、遗传背景清晰, 基因操作简便、能量供应成本低