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Chinese Science Bulletin, Volume 62 , Issue 33 : 3851-3860(2017) https://doi.org/10.1360/N972017-00419

In vitro synthetic biology: Cell-free protein synthesis

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  • ReceivedApr 18, 2017
  • AcceptedAug 31, 2017
  • PublishedSep 27, 2017

Abstract


Contributions statement

同等贡献

Equally contributed to this work


References

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  • Figure 1

    (Color online) Evolution of the protein synthesis from cell factory system to CFPS

  • Figure 2

    (Color online) The development history of CFPS

  • Figure 3

    (Color online) Overview of CFPS system: production formats, category of cell extracts, category of proteins produced and application fields

  • Table 1   Pros and cons of four types of cell extracts

    细胞提取物

    优点

    缺点

    大肠杆菌

    蛋白产量高、提取物制备简便、遗传背景清晰, 基因操作简便、能量供应成本低

    缺少翻译后修饰手段

    麦胚

    合成真核蛋白范围广、蛋白产量高

    产量低、制备耗时长且复杂、基因操作困难

    昆虫细胞

    细胞易破碎且快、可进行真核特异性翻译后修饰

    制备耗时长且成本高、基因操作困难

    兔网织红细胞

    细胞易破碎且快、可进行真核特异性翻译后修饰

    蛋白产量低、动物组织处理复杂、蛋白合成范围窄、基因操作困难、背景mRNA多