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SCIENCE CHINA Life Sciences, Volume 62 , Issue 9 : 1178-1193(2019) https://doi.org/10.1007/s11427-018-9456-x

Alterations in intestinal microbiota of colorectal cancer patients receiving radical surgery combined with adjuvant CapeOx therapy

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  • ReceivedSep 6, 2018
  • AcceptedDec 12, 2018
  • PublishedFeb 19, 2019

Abstract


Acknowledgment

This work was supported by the National Natural Science Foundation of China (81230057, 81372615, 81472262 and 81200264), the Emerging Cutting-Edge Technology Joint Research Projects of Shanghai (SHDC12012106) and Tongji University Subject Pilot Program (162385), Lijieshou Intestinal Barrier Foundation (LJS-201701), Specialized Research Fund for the Combine Traditional Chinese and Western Medicine in General Hospital of Shanghai (ZHYY-ZXYJHZX-1-201704).


Interest statement

The author(s) declare that they have no conflict of interest.


Supplement

SUPPORTING INFORMATION

Figure S1 Community richness and diversity index of the preoperative (Group B), postoperative (Group A0) and post-chemotherapy (Group A1–5) groups. A1–5 represent samples collected after the first to fifth cycles of chemotherapy, respectively.

Figure S2 Comparison of gut microbiota between preoperative (Group B) and post-chemotherapy (Group A1–5) groups. The dominant phyla (A) and genera (B) in these two groups. (C) Wilcoxon rank-sum test results in the two groups. A1–5 represent samples collected after the first to fifth cycles of chemotherapy, respectively.

Figure S3 The relative abundance of the Clusters of Orthologous Groups of proteins (COG) for predicted genes.

The supporting information is available online at http://life.scichina.com and http://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.


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  • Figure 1

    Comparison of gut microbiota between preoperative (Group B) and postoperative (Group A0) colorectal cancer patients. The dominant phyla (A) and genera (B) in these two groups. C, Cladogram of linear discriminant analysis (LDA) coupled with effective size measurement showing differentially abundant genera. D, Histogram of LDA scores for differentially abundant genera. E, Plot of principal co-ordinate analysis (PCoA) scores based on the relative abundance of operational taxonomic units (OTUs; 97% similarity level). Each symbol represents a sample.

  • Figure 2

    Comparison of gut microbiota between pre-chemotherapy (Group A0) and five cycles (groups A1–5) of chemotherapy groups. A, Plot of unweighted UniFrac Principal Co-ordinate Analysis (PCoA) scores based on the relative abundance of operational taxonomic units (OTUs; ≥97% similarity level). Each symbol represents a sample. B, Analysis of similarity within and between Group A0 and groups A1–5. C, Genus level heatmap based on unweighted UniFrac distance of all the samples in the preoperative (Group B), Group A0, and groups A1–5. The (D) dominant genera and (E) phyla in the seven groups and their relative abundance are listed. F, The top 30 prevalent bacterial genera identified in the seven groups with relative abundance denoted by circle size and colors representing different phyla.

  • Figure 3

    (Color online) Real-time quantitative polymerase chain reaction (PCR) for Dorea, Ruminococcaceae_UCG−010, Streptococcus, Enterobacteriaceae_unclassified, Prevotella_9, Mogibacterium, Roseburia, Bacteroides, Butyricicoccus, Enterococcus, Veillonella, Lactobacillus, and Parabacteroides. Groups included the pre-surgery Group B, pre-chemotherapy Group A0, and groups A1–5 during stages of chemotherapy. *, P<0.05.

  • Figure 4

    The predicted function and network construction of microbiota in preoperative (Group B), pre-chemotherapy (Group A0) and five cycles of chemotherapy (groups A1–5). A, Predicted gene functions in preoperative and postoperative groups in the L2 categories. Predicted gene functions in pre-chemotherapy and the (B) first, (C) fourth, and (D) fifth cycles of chemotherapy in the L3 categories. E, Co-occurrence networks of the top 50 bacterial genera in the seven groups. Solid and dotted lines indicate positive and negative correlations, respectively.

  • Table 1   Clinical characteristics of colorectal cancer patients (mean±SD) in the present study

    Group

    Preoperative group

    Postoperative group

    Chemotherapy group

    Male/female

    12/7

    7/3

    33/12

    Age (years)

    62.53±1.92

    62.60±3.27

    61.04±1.33

    BMI (kg m–2)

    23.89±0.87

    21.73±1.22

    22.57±0.65

    TNM stage (%)*

    II

    6 (31.58)

    3 (30.00)

    13 (28.89)

    III and IV

    13 (68.42)

    7 (70.00)

    32 (71.11)

    Tumor site (%)

    Ascending colon

    1 (5.26)

    2 (20.00)

    8 (17.78)

    Transverse colon

    1 (5.26)

    0 (0.00)

    1 (2.22)

    Descending colon

    2 (10.53)

    2 (20.00)

    3 (6.67)

    Sigmoid colon

    5 (26.32)

    2 (20.00)

    18 (40.00)

    Rectum

    10 (52.63)

    4 (40.00)

    15 (33.33)

    BMI, body mass index; SD, standard deviation; TNM, tumor, lymph node, and metastasis; GI, gastrointestinal. *, TNM staging was based on the standards of the American Joint Committee on Cancer.

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